FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l04_n02_D1S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l04_n02_D1S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28306470
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGGG97700.014.77284539
CGTGACT27400.013.276342
GGGGGCC163050.013.17626544
ATTCGGG33850.012.95162537
GTAGAAC46750.012.89059711
TTCGGGG83450.012.44671338
TTGGGGG137800.012.05643539
GGGGAAA162650.011.40657122
GGGGCCG111500.011.10524545
GGGGGGC324750.010.82345543
GGGGGAA250650.010.82016921
TCAGGTA59050.010.5240447
TGGGGGG254050.010.19369440
TTTGGGG104350.010.12615238
GCGTGAC37800.010.1053791
ATTTCGG27300.010.04751236
CTCGAAT27300.010.0179851
TTTCGGG36700.09.92423937
GTACTCG53750.09.8240444
TAGAACA61800.09.75281612
Produced by FastQC (version 0.11.5)