FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l04_n02_D1S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l04_n02_D1S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26703731
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT27200.013.8722412
GTAGAAC42000.012.95656911
GCGTGAC34650.011.6760841
TCAGGTA54950.010.3262557
GTACTCG67500.010.2303014
TAGAACA55500.09.84683712
TACTCGA68550.09.8021645
AGTACTC73750.09.6764493
GGTAGAA58550.09.48613110
CAGGTAG59950.09.422378
CTCGAAT24300.09.0222091
GGAAAAT115300.08.9976851
GTGACTC45250.08.6840363
AAAATCC86450.08.5945233
GAATCGA45300.08.4687924
CTAGCAC47350.08.40704314
AGATCAG98950.08.3014964
TATAAGG31550.07.861525
CTATAAG31300.07.85874034
GCTCTAG53750.07.82324811
Produced by FastQC (version 0.11.5)