FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l04_n02_D1S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l04_n02_D1S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29899166
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT26500.013.2153962
GTAGAAC45300.012.21109211
GTACTCG70450.010.6667564
TCAGGTA58700.010.20371157
TACTCGA72050.010.045765
GCGTGAC38200.09.88120651
AGTACTC81700.09.3982983
TAGAACA61600.09.23673712
CTCGAAT26250.08.9549031
GGAAAAT126250.08.8977841
GGTAGAA64050.08.81186510
GTGACTC44650.08.8007593
AAAATCC96600.08.5331253
CAGGTAG68050.08.366858
AATCGAA58550.08.3568885
GAATCGA44800.08.0091834
AATTCAC43400.07.8451095
TATAAGG32700.07.79188355
AGATCAG110550.07.736594
GAATTGA76050.07.65578274
Produced by FastQC (version 0.11.5)