FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l04_n02_D1S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l04_n02_D1S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25253221
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT23300.012.4121382
GTACTCG51550.09.8934454
GCGTGAC32250.09.7413441
GGAAAAT110850.09.3585981
GTAGAAC34450.09.268661511
TACTCGA54500.09.1019785
GAATCGA41000.08.42122654
GTGACTC38400.08.2919883
AGTACTC65250.08.2023553
AAAATCC79900.07.8289543
TTAGGGT23300.07.82761577
AATCGAA50400.07.78445675
CTAGCAC40950.07.74385614
AAATCCT88000.07.51368054
GCTCTAG44350.07.4531811
GAATTGA64200.07.4519274
TCAGGTA47750.07.4504777
ATTAGGG24400.07.38759146
CGTAGGT13100.07.38133535
AGAACGG127150.07.2285113
Produced by FastQC (version 0.11.5)