FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l04_n02_D1S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l04_n02_D1S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28916542
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT27150.013.5648872
GCGTGAC37350.011.1346631
GTACTCG63900.010.38289454
GTAGAAC39600.010.27771111
TACTCGA65200.09.9605755
ACTTTCG35100.09.5465519
AGTACTC76600.09.4929913
GGAAAAT130500.09.4565331
AAAATCC97100.09.1400653
AATCGAA60000.08.9446895
GTGACTC43000.08.9280473
GCCCTAT43150.08.80675610
GAATTGA77200.08.5064364
GAATCGA43200.08.4625894
GCTCTAG50800.08.32160711
TCAGGTA53950.08.1806577
CTAGCAC46600.08.15623814
TCCGTTA69100.08.07125219
AAATCCT101100.08.0580144
ATAGGGG41600.08.05476134
Produced by FastQC (version 0.11.5)