FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l04_n02_D1S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l04_n02_D1S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26960667
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCTCCTGAAGCAGATCGAGTACCTGCTCCGTTCCAAGTGGGTGCCCTGC277450.10290917505861409No Hit
CTCCCACCTTGTTTCGTGTGTTCCCAGTTTGTTCATCGTCTTTTGATTTTA273950.10161098759166455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGA34750.09.3513314
TATGCAG101200.09.0015519
TCAATCG27150.08.982783
AGTCTAG22700.08.8346126
TAGTCGT23100.08.76212616
TACGGGT53400.08.7612279
TGTATGC90750.08.7585747
GTATGCA95250.08.6003018
TTACGAC42000.08.5704929
AATCGAA51950.08.5508345
GCTGTAT87550.08.4735925
AGCTGTA93800.08.4684824
TGTTTCG150900.08.34473210
CTCTATA43500.08.2140545
CTGTATG94450.08.2069126
AACTCGC76650.08.0311595
GTTTCGT155800.08.02465811
TTTCGTG158700.08.0208312
ACCTTGT169700.07.90057046
TAAGAGT90600.07.862904544
Produced by FastQC (version 0.11.5)