FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l04_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l04_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18781869
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG13800.036.48952515
CGTCTGA14200.035.3033416
CACACGT15650.032.17221512
CACGTCT16100.031.41611514
ACACGTC17450.029.5009413
TCGGAAG20100.028.2986283
ATCGGAA20600.027.9417112
CAGTCAC11750.027.82436427
AGCACAC19200.026.34077510
CCAGTCA13400.026.08128426
GATCGGA22300.025.8226281
CGGAAGA23350.024.6486994
GCACACG20550.024.5009811
CTCCAGT21750.021.8502224
AACTCCA23900.020.60813122
GAACTCC29100.017.38637721
AGAGCAC30300.016.9137338
AGACCGA4750.016.6159532
AGTCACT3950.016.55046528
CTGAACT31250.016.04790519
Produced by FastQC (version 0.11.5)