FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l04_n01_D1S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l04_n01_D1S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22798810
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT36050.022.90999842
ACGTCTG40250.022.74355915
AGTCACG39800.022.74078828
TCGTATG39550.021.28334443
CGTCTGA43350.021.22091516
CGTATGC45000.018.90671244
TCGGAAG52250.017.9080143
ATCGGAA52700.017.413962
GTCACGA55600.016.48094729
CACGTCT58300.016.12637114
CAGTCAC58550.016.03520627
CACACGT60950.015.42519112
ACACGTC71050.013.51738613
TCTCGTA66200.013.15346141
ACGATCA73900.012.61399732
TCACGAT76750.011.99796130
GAGCACA84100.011.9547239
GTCCTAT24850.011.68363743
GATCGGA80600.011.6393071
CCAGTCA81600.011.56106526
Produced by FastQC (version 0.11.5)