FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l04_n01_D1S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l04_n01_D1S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25462775
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC426570.16752690938045833TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG106150.032.82242615
CGTCTGA109000.032.0467716
TCGGAAG118200.029.9523453
ATCGGAA119400.029.4635722
CTCGTAT75700.029.1634242
TCGTATG75200.028.79139543
GTCACAC121600.028.38350129
CGTATGC72900.028.2187544
CACGTCT128500.027.34112214
ACACGTC134500.026.22178313
CACACGT134750.026.13944212
AATCTCG104800.025.6984539
CAGTCAC133750.025.53593427
CACACTT140300.024.5376931
AGTCACA140850.024.28053728
GATCGGA147100.024.1183661
TCTCGTA97250.023.02093141
CGGAAGA156700.022.7942374
CCAGTCA151850.022.68488526
ACACTTG153450.022.67003832
Produced by FastQC (version 0.11.5)