FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l04_n01_D1S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l04_n01_D1S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23501528
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC344940.14677343532726894TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG69350.031.03896715
CGTCTGA73200.029.43718116
CTCGTAT61750.028.24269542
ATCGGAA79600.027.2691572
TCGTATG64700.025.9138143
TCGGAAG84000.025.840393
CACGTCT86300.025.1250614
CACACGT87800.024.61880112
CGTATGC67250.024.49705144
ACACGTC94900.023.15630113
CAGTCAC94400.022.61091627
GATCGGA101450.021.6662671
AGTCACC101450.021.28371628
CGGAAGA108300.020.208564
TCTCGTA88650.019.9744841
GTATGCC87650.019.28357945
CCAGTCA116750.018.20522326
AGCACAC120300.018.19220410
TCCAGTC119300.017.94327425
GCACACG122600.017.924311
Produced by FastQC (version 0.11.5)