FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l04_n01_D1S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l04_n01_D1S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28306470
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC3554181.2556069336798266TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT437650.039.96405842
TCGTATG435150.039.6744143
CGTATGC435500.039.1327844
TATCTCG479700.038.1693739
AGTCACG504900.037.9126128
GTATGCC456000.037.43327745
ACGTCTG520900.037.32037415
CGTCTGA523400.037.12492416
GTCACGC518700.036.97339629
TCTCGTA476150.036.96713341
CGCCAAT519400.036.66224733
ATCGGAA542050.036.371712
ACGCCAA523200.036.35243232
TCGGAAG542500.036.34093
CACGCCA533200.035.8389331
CACGTCT544150.035.7958614
CAGTCAC537450.035.72558627
CACACGT546700.035.71497712
GCCAATA533750.035.5452734
ATATCTC526800.035.53190238
Produced by FastQC (version 0.11.5)