FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l04_n01_D1S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l04_n01_D1S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26703731
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG52350.025.86563715
TCGTATG52050.025.07805443
CGTCTGA55000.024.5784916
CTCGTAT52850.024.39830242
CGTATGC61250.021.56883644
TCGGAAG65300.021.0809293
ATCGGAA68200.020.2509442
GTCACAC68000.019.76703829
CACGTCT72700.019.49165314
CACACGT76150.018.4902212
CAGTCAC78350.017.55826627
AGTCACA81950.016.4021828
ACACAGT84400.016.06015632
TCTCGTA82700.015.99739941
ACACGTC91500.015.92929313
CACAGTG85800.015.55984833
AGTGATC84700.015.53850936
CCAGTCA97450.014.0243626
GTATGCC99700.013.81508145
AGCACAC103300.013.80471510
Produced by FastQC (version 0.11.5)