FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l04_n01_D1S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l04_n01_D1S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29899166
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC569400.19044009454979446TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG103000.032.9090215
CGTCTGA105250.032.3123516
CTCGTAT90700.030.81579442
TCGTATG92200.030.46339443
TCGGAAG118950.029.0257593
ATCGGAA120300.028.5322362
CGTATGC96550.028.32241444
AGTCACT118200.028.17223228
ACTGACC126750.026.25615932
CACGTCT131700.026.16407614
CACACGT133650.025.57995412
CAGTCAC133250.025.31146827
AATCTCG123550.025.05785439
ACACGTC141350.024.50514613
GTCACTG139600.024.14387129
TCTCGTA123550.023.16534241
CACTGAC148000.022.50110831
GATCGGA155850.022.2583371
GACCAAT149400.021.9859835
AGCACAC159300.021.71529210
Produced by FastQC (version 0.11.5)