FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l04_n01_D1S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l04_n01_D1S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25253221
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC304260.12048364048293086TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG57800.030.27572415
ACTTAGG58150.029.54060432
CGTCTGA61700.028.3984816
CTCGTAT55700.027.6369842
TCGTATG56950.026.28234143
TCACTTA68500.025.23925230
ATCGGAA72800.024.4403152
TTAGGCA70550.024.33879734
TCGGAAG73150.024.2612083
CACTTAG72650.024.04706631
CGTATGC62800.023.6913644
GTCACTT73350.023.5397129
AGTCACT73450.023.4770128
CACGTCT77350.022.9434914
CTTAGGC76250.022.5853833
CACACGT85200.020.82948312
CAGTCAC85700.020.62036727
ACACGTC92400.019.76636713
TCTCGTA86200.018.35135341
TAGGCAT98000.017.70081335
Produced by FastQC (version 0.11.5)