FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l04_n01_D1S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l04_n01_D1S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28916542
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG51450.025.3118315
CGTCTGA53450.024.40678816
CTCGTAT48050.024.26040642
TCGTATG49500.023.09743743
CGTATGC53200.021.449444
TCGGAAG65100.020.4885653
ATCGGAA64350.020.4131682
CACCGAT63950.019.96366131
GATGTAT66950.018.98622535
ACCGATG68150.018.86597332
CACGTCT71300.018.86435714
TATCTCG67650.018.39212439
CACACGT76450.017.74052212
CAGTCAC80300.016.40264727
ACACGTC88300.015.38532213
AGTCACC89300.015.37968728
GTATCTC87650.014.93874438
TCTCGTA82550.014.71879541
AGCACAC97850.014.596189510
TGTATCT95250.013.79397237
Produced by FastQC (version 0.11.5)