FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l04_n01_D1S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l04_n01_D1S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26960667
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC750380.2783239746998841TruSeq Adapter, Index 1 (100% over 51bp)
CGGAGCTTTCATAGATTTCATTCACATATGCCTCACCCAACAACATATAGT431300.15997378699866735No Hit
CCGGAGCTTTCATAGATTTCATTCACATATGCCTCACCCAACAACATATAG300970.111632994836515No Hit
GGCAGCTTCCACATGGTCCAGTACCTGCCGTCGTAGTATCCAGGGGACCTG284040.10535347660352765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG115700.036.0809215
CGTCTGA118500.035.20939316
TCGTATG108350.034.83500743
CTCGTAT105550.034.3063842
CGTATGC109750.034.1460144
ATCGGAA130850.032.1795852
TCGGAAG133700.031.6781023
CACGTCT138600.030.37914714
CACACGT141500.029.9152712
ACACGTC145600.028.99585213
GATCGGA147750.028.6103061
GTCACAT145450.028.37286429
CAGTCAC148050.027.85953927
AGTCACA149050.027.59694528
TCACGAT147800.027.20184135
GTCTGAA156700.026.64047617
GTATGCC147950.025.81687245
ATCACGA161400.025.4753534
TCTCGTA146250.025.03166841
CGGAAGA172800.024.7444954
Produced by FastQC (version 0.11.5)