FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l03_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l03_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18636126
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN486870.26125064833753536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT9050.024.235442
AGTGTAG5050.022.71556929
TCTCGGT5050.022.25583337
GTGTAGA5150.021.40097830
GAGTGTA5200.020.76264628
TAGATCT5550.020.66305433
GTAGATC5850.019.22047632
TGTAGGG6000.019.1114718
GTGTAGG6050.018.95490317
TCGTGTA6200.017.41050115
TCCCTAC6150.016.8286029
TTCCCTA6450.016.057698
TACACGA6100.015.85263713
TAGGGAA7150.015.72254120
GTAGGGA7300.015.09193319
TGTAGAT7300.014.78903331
GATCTCG8750.014.38907635
CTCGGTG8850.014.22304138
AGCGGTT7350.014.07424810
AACCGCT3950.013.66581431
Produced by FastQC (version 0.11.5)