FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l03_n02_A1S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l03_n02_A1S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19380970
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN341340.17612121581117973No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC28050.012.58805311
TCAGGTA37200.010.414217
GTACTCG47650.09.8104024
TACTCGA48450.09.5461375
AGTACTC51800.09.51083853
GGAAAAT85650.09.4800991
GAATTGA49750.09.1693314
CAGGTAG40950.09.0699828
GGTAGAA40000.08.93954510
TAGAACA39250.08.93874912
CGTGACT16900.08.8293542
AAAATCC64850.08.2242093
GAATCGA33700.08.0413824
AATTGAC34150.07.92789945
TGGGGGG61150.07.71861440
AGATCAG70100.07.570584
TCGGGGG44300.07.35696639
CGAGAAT28300.07.27278471
GGTAGGC19250.07.25440457
AGAGATC77450.07.239392
Produced by FastQC (version 0.11.5)