FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l03_n02_A1S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l03_n02_A1S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23657364
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN414820.17534497926311657No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAATC104250.08.8052422
AATCCTA53200.08.5670125
GGGGGGC116800.08.291615543
AATTCAC47250.08.1655215
GGAAAAT107700.08.0643231
TAATCGA40900.07.89590024
AAAATCC109000.07.85835933
GGGGGCC83900.07.766051344
ATCCTAT46450.07.7636496
TCGGGGG61950.07.582875339
AATCGAG66100.07.4412295
ATAATCG34950.07.307193
GAAAAAT133700.07.27427051
CTTGAAT71600.07.26552871
TTTCGGG25800.07.144394437
GGGGAAT60800.07.03089571
CGGGAAT24600.06.98764661
GGCGAAT27650.06.9530641
TCGAAAC42400.06.947132618
AATCGTA14300.06.9423465
Produced by FastQC (version 0.11.5)