FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l03_n02_A1S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l03_n02_A1S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14090550
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN244500.1735205510075902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC23600.010.95884511
TCAGGTA29600.09.1311627
CAGGTAG30300.08.8403618
GGAAAAT62050.08.3843411
GGTAGAA31700.08.15840310
CGTAGGT7551.8189894E-128.04075535
TAGAACA33250.07.981305612
AGGTAGA34250.07.8820439
AGAGATC61350.07.81266162
AATTCAC24850.07.8079475
AATCGAA31750.07.6033485
AAATTGA54900.07.5275694
CGGGAAT11350.07.3737561
AAAAATC52400.07.29177242
CGGAAAT16150.07.2830571
AGATCAG58400.07.26977544
CGAGAAT22450.07.25435921
TATAGAA23100.07.24607231
GGGGAAT33450.07.16791151
GTACTCG17350.07.1587834
Produced by FastQC (version 0.11.5)