FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l03_n02_A1S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l03_n02_A1S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22546348
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN397600.1763478502150326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC36700.011.33662311
CGTGACT22700.010.6574992
CTCGAAT25550.09.9152411
TCAGGTA46150.09.8103857
TAGAACA47250.08.90059112
CAGGTAG47900.08.7411438
GGTAGAA49750.08.63397110
GCGTGAC31450.08.6305161
GGAAAAT94700.08.4791931
AATCGAA49650.08.2706995
GTACTCG46300.08.2434124
GAATTGA60300.08.2021574
AAAATCC75250.07.92888743
GAATCGA41750.07.8976464
GTGACTC32300.07.8366473
TACTCGA48350.07.6997665
AAAAATC74750.07.65255982
AGATCAG85750.07.5587344
AGTACTC55400.07.54704243
TACGCAC10150.07.526014345
Produced by FastQC (version 0.11.5)