FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l03_n02_A1S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l03_n02_A1S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24528422
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN436370.17790382112636516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC34650.012.78649411
CGTGACT26450.011.2828842
GCGTGAC30350.010.8056931
TCAGGTA48550.010.2531937
GTACTCG62500.09.68353754
TACTCGA62500.09.6026545
AAAATCC84350.09.5382353
AGTACTC68400.09.2843893
TAGAACA48100.09.25785612
CAGGTAG49600.09.1675398
GGAAAAT112250.09.0268241
GGTAGAA50800.08.98688710
GTGACTC37850.08.5383363
CTAGCAC39900.08.342154514
GAATTGA60850.08.313174
TCCGTTA19750.08.31087219
AATTGAC41400.08.0658665
GATCTAG37100.07.96685745
AAATCCT87200.07.89882664
AAAAATC80550.07.83087872
Produced by FastQC (version 0.11.5)