FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l03_n02_A1S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l03_n02_A1S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26959356
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC35500.010.89644811
TACTCGA72900.010.7705435
CGTGACT26250.010.7661212
GTACTCG73550.010.6853914
AGTACTC79650.010.186383
TCAGGTA46950.09.1631197
GGAAAAT116500.09.0669681
CTCGAAT28150.08.5975861
AATCGAA60050.08.5665855
GCGTGAC36550.08.4782131
GAATCGA47150.08.3820494
ACTCGAG106250.08.0181916
GAATTGA70000.08.001064
AAAATCC85800.07.87580873
TAGAACA50000.07.87144612
GGTAGAA50400.07.80875610
CAGGTAG52500.07.80344968
AGCCTAG21950.07.787960525
TATAAGG28000.07.65511045
GTGACTC39750.07.56180953
Produced by FastQC (version 0.11.5)