FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l03_n02_A1S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l03_n02_A1S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25357257
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT26800.012.0649812
GTAGAAC31450.010.29716211
GCGTGAC33650.010.2849451
GTGACTC37900.09.0646323
CTAGCAC39050.08.98421514
GAATTGA71550.08.5860524
CTCGAAT25650.08.4660231
TCAGGTA43450.08.4497717
CCGTAGA13950.08.37896238
GTACTCG66700.08.2622494
AATCGAA61850.08.2448075
GCTCTAG43850.08.20589711
GGAAAAT110450.08.19199851
GAATCGA50950.08.1565764
TACTCGA66950.08.1559885
AGTACTC70250.08.0755713
AAAATCC85800.07.79740763
AATTGAC48250.07.71610985
CAGGTAG43900.07.64003568
AAATCCA97100.07.58285334
Produced by FastQC (version 0.11.5)