FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l03_n02_A1S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l03_n02_A1S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24767146
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN428620.17305990766962007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT25400.011.6604572
GTAGAAC29800.010.41480811
GCGTGAC31250.010.2769231
ACTTTCG33450.010.21726819
AGTACTC71300.09.7942263
GTACTCG71000.09.5102014
CTCGAAT23550.09.507561
TACTCGA69400.09.4280265
ATAGGGG38500.09.22816534
GAACGCT29350.09.1160921
GCCCTAT41800.08.98498210
AATCCGC44100.08.9021095
GGAAAAT100600.08.7453341
ATTAGGG42000.08.4783636
TTAGGGT41300.08.3444727
AATCGAA57700.08.2897665
AGTCTCG33100.08.1115015
TGCTAAC78700.08.1079844
GTGACTC39250.08.0610113
GAATTGA66150.08.0567344
Produced by FastQC (version 0.11.5)