FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l03_n02_A1S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l03_n02_A1S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23931659
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN419300.17520724325881462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT24550.012.8013682
GCGTGAC30900.011.0529961
GTGACTC34750.09.5604663
GTAGAAC30700.09.45009311
GTACTCG65750.09.10234
AGTACTC70250.08.9114823
TACTCGA68200.08.8000535
GGAAAAT112550.08.6414071
GAATTGA64150.08.6252244
AAAATCC83600.08.596813
GCTCTAG41950.08.36329511
AATTGAC43350.08.1193925
TATAAGG30450.08.0024785
CTAGCAC40400.07.960500214
TCAGGTA43600.07.90410857
CTCGAAT22900.07.9016181
ATTGACC40850.07.88965946
AAATCCT86950.07.7660934
CTATAAG30700.07.72417164
AATCCGC29650.07.6096265
Produced by FastQC (version 0.11.5)