FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l03_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l03_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18636126
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG9000.029.97451615
CGTCTGA8950.029.89078716
CACGTCT9800.027.7569414
CACACGT10100.026.484412
ACACGTC11100.024.50612613
AGCACAC11950.022.7605110
TCGGAAG15950.022.1329773
ATCGGAA16850.020.5512392
GCACACG13300.020.28122111
GATCGGA17500.020.1435871
CAGTCAC8750.019.82190327
CCGAGAC5450.019.42513529
CGGAAGA18450.019.0120544
GAGACCG5750.018.4124931
CCAGTCA10250.018.02243226
AGACCGA6150.017.58106232
CTCCAGT14950.016.26477224
AACTCCA16350.016.23135822
ACCGATC6650.015.23736934
AGTCACT3500.014.80207228
Produced by FastQC (version 0.11.5)