FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l03_n01_A1S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l03_n01_A1S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19380970
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC484240.24985333551416672TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT71800.032.9375442
ACGTCTG77850.032.40733715
CGTCTGA78950.031.8988516
TCGTATG75300.031.67560243
AGTCACG79050.031.14560328
CGTATGC78450.030.14566844
CACGTCT90350.028.32195714
TCGGAAG92600.027.8294143
CACACGT91950.027.78201712
GTCACGA89300.027.7218729
ATCGGAA93150.027.5697462
TCTCGTA95450.025.10373541
ACACGTC102250.024.95983313
GTATGCC100200.024.09487745
ACGATCA105500.023.53014232
TCACGAT105650.023.45303730
CAGTCAC107800.023.34048827
GATCGGA111700.023.1522791
CGGAAGA115200.022.682264
CCAGTCA113900.021.915326
Produced by FastQC (version 0.11.5)