FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l03_n01_A1S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l03_n01_A1S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23657364
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC547970.23162766570273846TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG113450.032.5645915
CGTCTGA113900.032.39644616
CTCGTAT90150.030.77481742
CGTATGC85550.030.74647944
TCGGAAG123100.030.5789663
TCGTATG89500.030.47043243
ATCGGAA126350.029.5981432
CACGTCT125200.029.5802514
CACACGT130900.028.2769812
GATCGGA135900.027.6805111
GTCACAC132400.027.33246629
ACACGTC136350.027.26027713
GTATGCC99400.026.73267245
GTCTGAA144800.025.71605517
CGGAAGA148700.025.5415254
AATCTCG125400.025.34949139
CAGTCAC146850.025.118227
AGTCACA151100.024.21792228
CACACTT155150.023.48519731
TCTCGTA120800.023.39280341
Produced by FastQC (version 0.11.5)