FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l03_n01_A1S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l03_n01_A1S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14090550
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT24800.020.04996942
TCGTATG29400.017.14248743
CGTATGC31150.015.96290644
ACGTCTG38350.014.83469315
CGTCTGA38700.014.75863316
CACACGT44600.013.26035212
ATCGGAA46050.012.3556812
TCGGAAG46850.012.3360533
CACGTCT47000.012.29585314
TCTCGTA40850.012.11638141
GTATGCC42550.012.05567345
GTCGGTT13700.011.79785251
ACACGTC52050.011.53490313
AGTCACC54250.010.99359128
CAGTCAC55950.010.65963527
CCCGACT14100.010.6558961
GATCGGA56350.010.2209631
CGGAAGA60300.09.9947814
TTAGCCG29100.09.8140798
CCAGTCA61800.09.61519826
Produced by FastQC (version 0.11.5)