FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l03_n01_A1S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l03_n01_A1S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22546348
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC356880.15828727561554537TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT57350.029.3073442
ACGTCTG63250.028.37073115
AGTCACG61700.028.26927628
TCGTATG60500.027.6326643
CGTCTGA66500.026.95037716
TATCTCG68000.025.2417339
GTCACGC70500.024.99601729
CACGTCT75600.024.12277214
ATCGGAA77000.023.539882
TCGGAAG78300.023.3204483
CACACGT78550.023.16034112
CGTATGC71400.023.03617344
CGCCAAT82000.021.3233433
ACGCCAA82900.021.20296532
ACACGTC87700.020.94838713
CACGCCA85500.020.74277931
CAGTCAC91350.019.98073227
TCTCGTA87150.019.64563441
TCACGCC91350.019.51258530
GATCGGA96950.018.9029451
Produced by FastQC (version 0.11.5)