FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l03_n01_A1S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l03_n01_A1S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24528422
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC362040.14760020029009613TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG61000.029.8225315
CTCGTAT59100.029.77078642
CGTCTGA61250.029.70080216
TCGTATG61100.029.12770843
CGTATGC70300.025.28387844
CACGTCT75750.024.63889514
TCGGAAG75750.024.4321923
GTCACAC75750.023.82917429
ATCGGAA78100.023.6690162
CACACGT83700.022.24577312
ACACGTC89950.020.77426313
AGTCACA87900.020.53538128
CACAGTG91750.019.74428733
CAGTCAC93950.019.71614827
AGTGATC91750.019.21894836
ACACAGT95050.019.15603632
TCTCGTA94400.019.08902741
GTATGCC95800.018.95227845
GATCGGA105300.017.8152331
CGGAAGA107900.017.5900884
Produced by FastQC (version 0.11.5)