FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l03_n01_A1S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l03_n01_A1S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26959356
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC293880.10900853863126404TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG65000.027.12224815
CGTCTGA65750.026.60766616
CTCGTAT56600.026.39614342
TCGTATG60100.024.89637243
AGTCACT75750.022.69883528
CGTATGC67300.022.09925844
TCGGAAG82050.022.0077293
ACTGACC78850.021.92108332
CACGTCT84200.021.52513514
ATCGGAA85150.020.969762
CACACGT89850.020.14726312
ACACGTC97750.018.63336213
AATCTCG89500.018.399539
CAGTCAC97250.018.3748827
CACTGAC97800.017.71988331
GTCACTG99750.017.57589329
CGGAAGA111200.016.4410554
GTATGCC94500.016.4281745
CCAGTCA107900.016.20851526
GACCAAT109400.015.81003135
Produced by FastQC (version 0.11.5)