FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l03_n01_A1S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l03_n01_A1S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25357257
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC410860.16202856641788976TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG68550.031.12164932
CTCGTAT65750.030.0476142
ACGTCTG73350.029.7686215
CGTCTGA75350.028.97848116
TCGTATG70050.028.01041443
CGTATGC73550.026.6164144
AGTCACT81650.026.26648128
TTAGGCA82350.026.08792734
TCGGAAG85250.025.982793
GTCACTT84450.025.39559629
TCACTTA85000.025.25774830
CACACGT87400.025.2160212
CACTTAG86550.025.0139731
CACGTCT90200.024.45693814
ATCGGAA90200.024.4086652
ACACGTC101750.022.03435713
CTTAGGC99200.021.73090733
CAGTCAC105900.020.6980327
TAGGCAT107700.020.00664135
GTATGCC100400.019.90092545
Produced by FastQC (version 0.11.5)