FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l03_n01_A1S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l03_n01_A1S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24767146
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG45600.025.34659415
CTCGTAT42400.025.2607742
CGTCTGA45800.025.08862116
TCGTATG44950.023.32719843
TCGGAAG56150.021.0652663
CGTATGC50550.021.01005644
CACGTCT58500.020.18016814
GATGTAT56650.020.16923735
CACCGAT57500.020.11833631
TATCTCG55700.020.03348739
ATCGGAA60150.019.3287352
CACACGT62200.019.12531312
ACCGATG63500.018.18176532
ACACGTC71100.017.01508113
CAGTCAC73200.016.5100927
AGTCACC81200.015.32684328
TGTATCT75500.015.2832737
TCTCGTA74400.015.18090541
GTATCTC77950.014.74793838
AGCACAC86550.014.08236210
Produced by FastQC (version 0.11.5)