FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l03_n01_A1S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l03_n01_A1S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23931659
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC376490.15731880518605085TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG64550.032.32703415
CTCGTAT61400.030.60251842
CGTCTGA68400.030.34308616
TCGTATG67650.029.07252343
TCGGAAG77600.027.2393423
ATCGGAA78450.026.7451212
CGTATGC74500.026.42967444
CACACGT83400.025.31824712
CACGTCT84750.024.8872714
GTCACAT87900.023.60617629
TCACGAT91750.022.35747135
ACACGTC95500.022.34482813
AGTCACA94650.022.0415828
TCTCGTA88850.021.49982341
CAGTCAC100150.021.16826827
GATCGGA102100.020.7743991
ATCACGA101250.020.53081334
AGCACAC108400.019.9358410
GAGCACA111100.019.410879
CATCACG108800.019.13134833
Produced by FastQC (version 0.11.5)