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        Note that additional data was saved in multiqc_data when this report was generated.

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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2019-04-10, 16:04 based on data in: /beegfs/mk5636/logs/html/CBY82ACXX/2

        General Statistics

        Showing 20/20 rows and 3/5 columns.
        Sample Name% Dups% GCM Seqs
        CBY82ACXX_l02_n01_N1S1
        68.5%
        52%
        24.7
        CBY82ACXX_l02_n01_N1S2
        70.6%
        52%
        29.5
        CBY82ACXX_l02_n01_N1S3
        69.0%
        52%
        28.6
        CBY82ACXX_l02_n01_N1S4
        69.7%
        52%
        28.5
        CBY82ACXX_l02_n01_N1S5
        69.9%
        52%
        28.4
        CBY82ACXX_l02_n01_N1S6
        68.9%
        52%
        29.4
        CBY82ACXX_l02_n01_N1S7
        65.4%
        51%
        23.9
        CBY82ACXX_l02_n01_N1S8
        68.8%
        52%
        25.5
        CBY82ACXX_l02_n01_N1S9
        67.3%
        52%
        22.1
        CBY82ACXX_l02_n01_undetermined
        90.5%
        45%
        16.8
        CBY82ACXX_l02_n02_N1S1
        69.7%
        54%
        24.7
        CBY82ACXX_l02_n02_N1S2
        72.2%
        54%
        29.5
        CBY82ACXX_l02_n02_N1S3
        70.5%
        54%
        28.6
        CBY82ACXX_l02_n02_N1S4
        71.0%
        54%
        28.5
        CBY82ACXX_l02_n02_N1S5
        71.3%
        54%
        28.4
        CBY82ACXX_l02_n02_N1S6
        70.0%
        54%
        29.4
        CBY82ACXX_l02_n02_N1S7
        67.0%
        53%
        23.9
        CBY82ACXX_l02_n02_N1S8
        69.7%
        54%
        25.5
        CBY82ACXX_l02_n02_N1S9
        68.7%
        54%
        22.1
        CBY82ACXX_l02_n02_undetermined
        89.3%
        46%
        16.8

        Lane 2 Demultiplexing Report

        Lane 2 Demultiplexing Report
        Total Read Count: Total number of PF (Passing Filter) reads in this library.
        Portion: The proportion of reads that represent the individual library in the entire Library Pool

        Showing 10/10 rows and 2/2 columns.
        Sample NameTotal Read CountPortion (%)
        undetermined_library
        16,791,050
        6.5
        N1S1
        24,681,264
        9.6
        N1S2
        29,487,747
        11.5
        N1S3
        28,649,731
        11.1
        N1S4
        28,460,088
        11.1
        N1S5
        28,429,607
        11.0
        N1S6
        29,429,469
        11.4
        N1S7
        23,937,989
        9.3
        N1S8
        25,510,129
        9.9
        N1S9
        22,103,460
        8.6

        Barcodes of Undetermined Reads

        Barcodes of Undetermined Reads
        We have determined the barcodes of your undetermined reads (reads containing a barcode that you did not encode in your metadata). Here are the top 20 barcodes belonging to the undetermined reads. The full list is available here.

        Showing 20/20 rows and 2/2 columns.
        Sample NameCountFrequency (%)
        CCCCCC
        103501.0
        0.6
        CGCCCC
        76398.0
        0.5
        TAGGCA
        72857.0
        0.4
        GCCCAA
        70514.0
        0.4
        CACCCC
        67153.0
        0.4
        CTCCCC
        65137.0
        0.4
        CGGCCC
        60866.0
        0.4
        GATGTA
        59567.0
        0.3
        CCCCCA
        58324.0
        0.3
        CGCCCA
        57557.0
        0.3
        TGCCCC
        56204.0
        0.3
        CAGCCC
        55123.0
        0.3
        CCGCTC
        54419.0
        0.3
        CCCCAC
        52512.0
        0.3
        CCGCCC
        48904.0
        0.3
        CCCACC
        48165.0
        0.3
        CCCCAT
        47525.0
        0.3
        ACTGAA
        47055.0
        0.3
        GCCCCC
        46788.0
        0.3
        GACCAA
        46787.0
        0.3

        Lane Statistics

        Lane Statistics

        Showing 1/1 rows and 4/4 columns.
        Sample NameTotal # of Single-End ReadsTotal # PF Reads% Undetermined % PhiX Aligned
        2.0
        272,348,352
        257,480,534
        6.5
        6.7

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

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        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

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        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

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        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

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        Sequence Length Distribution

        All samples have sequences of a single length (51bp).

        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

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        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        20 samples had less than 1% of reads made up of overrepresented sequences

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

        No samples found with any adapter contamination > 0.1%

        MultiQC v1.0.dev0 - Written by Phil Ewels, available on GitHub.

        This report uses HighCharts, jQuery, jQuery UI, Bootstrap, FileSaver.js and clipboard.js.