FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16791050
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN315620.18796918596514214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTCTT8900.023.1283152
AGTGTAG5250.020.98679429
TAGATCT5750.020.33430133
GTGTAGA5600.019.67511730
GTAGATC5600.019.27324132
GAGTGTA5800.018.60892328
TCGTGTA6900.017.5984115
TCTCGGT6150.017.54542437
TTCCCTA6050.016.3810468
TGTAGGG6900.016.29253618
TCCCTAC5350.015.9840099
GTGTAGG7650.015.28445417
GTAGGGA7600.014.79151119
TGTAGAT8050.013.68703931
TAGGGAA8550.013.14694920
TACACGA5350.012.60956613
TACGCAC1250.002250868612.6021299
GATCTCG10350.012.59880935
CTCGGTG9900.012.26167538
ATCGGAA11600.012.0899992
Produced by FastQC (version 0.11.5)