FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l02_n02_N1S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l02_n02_N1S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22103460
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN383540.1735203447786003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC31800.012.37586211
CGTGACT19550.09.9479072
GGTAGAA40200.09.78937210
TCAGGTA41300.09.7624997
TAGAACA41000.09.65368912
AGTCTCG16950.09.1861335
GAATCGA34600.09.0730694
CAGGTAG46250.08.8582758
AATCGAA48850.08.7769265
GTACTCG37200.08.7424874
CACTCGT34400.08.69318435
GAATTGA59700.08.6253374
ACTTTCG21700.08.60046219
GCCCTAT26750.08.32250910
GGAAAAT98400.08.1620581
GCGTGAC28750.08.1052381
ATCTAGC40050.08.1052156
CTCTATA36350.08.09744845
TTAAGTC22150.08.0655392
TACTCGA40450.07.92178775
Produced by FastQC (version 0.11.5)