FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l02_n02_N1S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l02_n02_N1S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25510129
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN436770.171214343918057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC41500.013.16797811
CAGGTAG54800.010.064038
TCAGGTA56750.09.962457
TAGAACA54650.09.87602112
GTACTCG46950.09.7650214
GGTAGAA54150.09.67596910
GGAAAAT123500.09.2145711
AAAATCC82400.08.9149323
TACTCGA51600.08.7467345
CGTGACT18150.08.7219112
AGATCAG97750.08.4793414
AGTACTC60400.08.2702243
AATCGAA50650.08.1979265
GAATCGA36950.08.129244
AATTGAC42500.08.0177715
GAATTGA65450.07.76400234
AAATCCT90650.07.6984534
CTCGAAT23100.07.5414151
AGGTAGA72150.07.51430039
AAAAATC79200.07.45256232
Produced by FastQC (version 0.11.5)