FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l02_n02_N1S7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l02_n02_N1S7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23937989
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN410050.17129676181236445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC41500.011.70486211
TCAGGTA51450.010.594187
GGTAGAA51450.09.39699910
CAGGTAG51400.09.3282588
TAGAACA53000.09.2499912
ATTAGGG33450.08.4912396
AGAACGG154900.08.463123
ACTTTCG24000.08.33854519
GAATCGA43900.08.2310574
AACGGCC110900.08.2206215
GGAAAAT103100.07.9654791
TTAGGGT33800.07.92989837
CGTCCAG125550.07.857388543
GATCTAG44000.07.8468185
ATCTAGC45350.07.7543246
AGTCTCG18650.07.7438335
CTCGAAT23400.07.7346041
ACGGCCG119500.07.67755846
GGAGAAT97900.07.67218971
GAACGCT22100.07.630743521
Produced by FastQC (version 0.11.5)