FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l02_n02_N1S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l02_n02_N1S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29429469
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC51900.014.47269811
TCAGGTA64500.012.39652257
CAGGTAG64550.011.71721658
GGTAGAA66750.011.48804510
TAGAACA66900.011.2949312
GTACTCG49350.09.7473244
AGATCAG117450.09.0949664
GAATCGA50000.08.8979344
AGGTAGA89850.08.6631639
AGTACTC63600.08.6355393
TACTCGA55400.08.5536415
GGAAAAT121550.08.487351
AATCGAA55800.08.4518855
AGAGATC132450.08.4514872
CGTGACT25050.08.3053882
AATCGAC37650.08.21101955
ATCAGGT135250.08.1999526
CACTCGT38100.08.14404535
CTCGAAT28900.08.0630921
CAGTGAG96800.07.94533717
Produced by FastQC (version 0.11.5)