FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l02_n02_N1S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l02_n02_N1S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28429607
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC47250.012.61288711
CGTGACT23800.012.1607862
GTACTCG53750.010.8395594
TCAGGTA60350.010.8237467
CAGGTAG62600.010.0686678
GGTAGAA59700.010.01966610
TAGAACA59750.09.898931512
TACTCGA57700.09.6985735
GCGTGAC35250.09.36928751
AGTACTC66000.09.3480633
CACTCGT44050.09.1879435
AAAATCC85000.08.853773
CTCTATA48100.08.68863545
AATCGAA57500.08.4765255
GGAAAAT122300.08.4158461
ACTCGTG48900.08.36778336
AATCCGC42100.08.3077145
ACTTTCG25000.08.27490619
GAACGCT29000.08.2187621
TCACTCG47700.08.1554334
Produced by FastQC (version 0.11.5)