FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l02_n02_N1S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l02_n02_N1S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28460088
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC42300.013.02551911
CGTGACT26800.012.0663772
TCAGGTA54800.010.6454357
GTACTCG55250.010.4228554
TAGAACA55700.010.01303812
GGTAGAA55500.010.00794710
CACTCGT52550.09.88375935
CAGGTAG58100.09.7635228
TACTCGA59550.09.4732165
GCGTGAC36400.09.2601411
CTCTATA58150.09.15753545
AGTACTC68500.09.0075223
ACTCGTG58450.08.92376436
GGAAAAT124250.08.8849731
TCACTCG59400.08.5932534
GAATCGA42400.08.468574
GAATTGA74600.08.2339624
GCGGAAA75500.08.1799081
GTGACTC45450.08.1553613
AAAATCC84750.08.0271253
Produced by FastQC (version 0.11.5)