FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l02_n02_N1S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l02_n02_N1S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28649731
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGACT26850.013.39028552
GTAGAAC44150.010.79882111
GTACTCG62850.010.0242764
GCGTGAC39900.09.8647861
TACTCGA66300.09.4273055
TCAGGTA56150.09.1456647
AGTACTC77150.08.6123753
AAAATCC92200.08.5547243
TAGAACA56400.08.45334812
GGTAGAA58150.08.3918210
CACTCGT44550.08.27727835
GAATCGA46850.08.2421524
CAGGTAG59700.08.2197838
GGAAAAT133950.08.1736111
GTGACTC47900.08.1152973
AATTGAC46800.08.0517815
GCTCTAG51850.08.0240611
CTAGCAC49200.07.953613814
GAATTGA81350.07.8279064
AATCGAA60300.07.55884465
Produced by FastQC (version 0.11.5)