FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l02_n02_N1S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l02_n02_N1S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29487747
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC43550.012.03182511
CGTGACT27200.011.6395392
TCAGGTA55500.010.1454597
GCGTGAC37950.09.3595921
TAGAACA59400.08.97275212
GTACTCG56350.08.7769884
GAATTGA84200.08.6633484
GGAAAAT140350.08.4628361
GGTAGAA63350.08.44829310
AAAATCC95900.08.4374713
CAGGTAG63500.08.43693358
ACTTTCG21650.08.41278719
CTCGAAT28600.08.3060081
TACTCGA59200.08.2717085
AATCGAA64450.08.1231075
AGTACTC69500.07.93510873
GTGACTC46250.07.6236113
AATTGAC52750.07.57195435
GGGAAAT69750.07.49267441
GAATCGA50300.07.45307454
Produced by FastQC (version 0.11.5)