FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l02_n02_N1S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l02_n02_N1S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24681264
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAC31800.012.02235211
CGTGACT21500.010.7289552
GGAAAAT112350.08.9409211
GTACTCG41400.08.8372464
GAATCGA38550.08.6118264
AATTGAC40150.08.5989915
TCAGGTA45150.08.5802697
GGTAGAA45850.08.4359310
AAAATCC77650.08.3249313
TAGAACA45700.08.21803512
CTCGAAT23500.08.1832061
GAATTGA68100.08.1581234
GCGTGAC32350.08.1125271
CAGGTAG46950.08.1025468
CGCGTAT22250.08.08544310
CACTCGT41000.08.06149435
TACTCGA45600.07.91765835
AATCGAA55750.07.77137765
CTCTATA43700.07.712520645
ACTCGTG43250.07.69340936
Produced by FastQC (version 0.11.5)