FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l02_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l02_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16791050
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG11350.035.66953315
CGTCTGA11750.034.45525416
CACACGT12700.031.86811312
CACGTCT12900.031.20902814
ACACGTC14300.028.7827313
ATCGGAA18400.026.0375062
TCGGAAG18750.025.3092173
CAGTCAC11450.024.00026327
AGCACAC17500.023.64305310
GCACACG17400.023.26006111
GATCGGA21000.022.711041
CCAGTCA13150.022.26690326
CGGAAGA22600.021.0982114
AACTCCA20550.019.49253822
AGACCGA4950.019.1114732
CTCCAGT18750.018.97464624
GAGACCG5450.017.77188931
CCGAGAC5550.017.04582229
ACCGATC6100.015.87054134
AGAGCAC27050.015.7947018
Produced by FastQC (version 0.11.5)