FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l02_n01_N1S9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l02_n01_N1S9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22103460
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC510570.23099098512178637TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA81400.033.29873716
AGTCACG80050.033.1846828
TCGTATG76950.033.10462643
ACGTCTG82850.032.85171515
CTCGTAT77400.032.3594542
CGTATGC81850.031.01331544
ATCGGAA95050.028.7340322
TCGGAAG95000.028.7237193
GTCACGA93650.028.48574429
CACGTCT98550.027.84635414
CACACGT100100.027.4604212
CAGTCAC102700.026.12900427
ACACGTC111600.024.8723113
TCTCGTA101650.024.5482241
GATCGGA114250.024.0282481
GTATGCC112650.023.01330845
TCACGAT118200.022.5880830
CCAGTCA118150.022.53991526
GAGCACA126850.022.3447599
ACGATCA121350.022.13114532
Produced by FastQC (version 0.11.5)