FastQCFastQC Report
Wed 10 Apr 2019
CBY82ACXX_l02_n01_N1S8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCBY82ACXX_l02_n01_N1S8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25510129
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC291970.1144525768568242TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG75550.029.89243915
CGTCTGA79650.028.32547816
CTCGTAT57700.027.34090642
TCGTATG59200.025.6599243
ATCGGAA91600.025.3946632
TCGGAAG91250.025.392063
GTCACAC88000.025.1211329
CACACGT93700.024.41454112
CACGTCT98750.023.48462514
CGTATGC65300.022.1947944
CAGTCAC103700.021.79542527
ACACGTC107650.021.54302813
CACACTT105300.021.18607731
AGTCACA105350.021.13353528
GATCGGA115150.020.1266141
AATCTCG95150.019.95669239
TCACACT115050.019.5274630
TCTCGTA82750.019.44221341
ACACTTG118250.019.1130232
CGGAAGA128700.018.3544674
Produced by FastQC (version 0.11.5)